临床前成像系统
面向科研的先进成像解决方案
从实时生物发光、荧光成像，到NIR-II / SWIR成像与数字放射自显影，Biospace Lab 研发高端成像平台，高灵敏、高精度且灵活地捕捉各类生物过程。
生物发光与可见光 / NIR-I
ORSO · ORSO Compact
生物发光成像・400 nm–900 nm・切伦科夫・实时成像
NIR-II / SWIR成像
ORIA
900–1700 nm・深层组织成像・纳米测温・实时成像
数字放射自显影
BetaIMAGER dFine · tRacer
高灵敏度・高分辨率・定量成像
实时生物发光与荧光成像
实时捕捉动态生物过程。
Biospace Lab 实时光学成像技术可对生物发光、荧光以及切伦科夫信号进行连续采集与定量分析，时间分辨率可达 50 ms以内。
单光子级灵敏度
检测超微弱生物信号。
实时定量分析
以 50 ms以内的时间分辨率追踪信号动力学变化。
活体动态生物成像
研究动态生物过程，支持对自由活动动物进行成像。
模块化设计
可随研究需求拓展成像功能。
两款实时光学成像平台，满足多样化科研需求，既可实现高端高度模块化成像，也可用于简洁化的生物发光与荧光成像应用。
ORSO
高端实时光学成像
本公司旗舰级实时光学成像系统，面向高要求的临床前研究开发。ORSO 兼具超高灵敏度、实时采集能力与模块化架构，可随科研需求持续升级拓展。
至多 10 只小鼠
时间分辨率＜50 ms
生物发光成像・荧光成像・切伦科夫成像
高度模块化，支持升级
精简型实时成像
面向需要小型化精简方案的实验室，ORSO Compact 将 Biospace Lab 实时生物发光与荧光成像技术集成于简易易用的平台。
至多 5 只小鼠
时间分辨率＜50 ms
生物发光成像・荧光成像・切伦科夫成像
小巧精简结构设计
哪一款 ORSO 更适合您？
两款实时成像平台，您可选择贴合自身科研需求的配置。
ORSO 因何出众？
增强型相机入射面捕捉到单个光子后，经增强会形成高亮光斑，便于 CMOS 识别与采集。
This technology makes it possible to count each photon individually emitted by the signal center. This method of quantification is called photon counting and leads to an almost perfect linearity as it sums each photon detected.
The photon counting technology combined with the high dynamic range of the optical chain allows the system to reach a global linear range over 106 and a linear range within a same image over 104.
The high sensitivity opens the way towards bioluminescence detection to a few cells in subcutaneous tissues and a few hundred cells in deep tissues (livers, lungs, pancreas…).
肺部转移瘤检测示例。即便经过手术切除，原发肿瘤与转移灶的信号强度仍相差 1000 倍。
肺部转移瘤检测示例。即便经过手术切除，原发肿瘤与转移灶的信号强度仍相差 1000 倍。
增强型相机入射面捕捉到单个光子后，经增强会形成高亮光斑，便于 CMOS 识别与采集。
该技术可对信号源发射的每一个光子单独计数。这种定量方式称为光子计数，通过累加所有探测到的光子，可实现近乎理想的线性响应。
光子计数技术搭配光学链路的高动态范围，使系统整体线性范围可达 10⁶，单张图像内线性范围可达 10⁴。6 and a linear range within a same image over 104.
超高灵敏度可实现皮下组织内少量细胞的检测，以及肝脏、肺、胰腺等深部组织内数百个细胞的检测。
凭借高时间分辨率与优异灵敏度，ORSO 从实验最初数秒起即可持续呈现光信号分布，并且可随时停止采集。 ORSO 系统的动态成像能力可精准、独立追踪信号动力学变化，即便信号微弱也不受影响。 在信号采集过程中和采集结束后，均可对每个感兴趣区域（ROI）的测量时段进行精准控制。 可依次为每只实验动物或各个解剖区域精准选取合适的信号测量窗口。
只有实时采集技术能够捕捉荧光素酶酶促反应动力学，从而实现精准定量。
定量分析可在合适的时间点执行。定量结果受荧光素体内分布影响（例如麻醉状态、进食情况、昼夜节律等）。每次采集时，应当在酶活性达到稳定、信号强度处于合适状态时完成信号定量。该阶段称为信号平台期。不同动物的信号动力学存在差异，主要原因是标记细胞分布位置不同；只有实时采集，才能为各个信号发射位点选择对应的定量时间窗口。
即便采用相同实验方案与条件，不同信号位点的动力学曲线依然存在差异，可靠的定量需要单独确定每一个位点的平台期。
上图展示 3 只小鼠的信号动力学曲线。小鼠皮下注射转染萤火虫荧光素酶（Fluc）基因的间充质干细胞。采集前，每只小鼠均腹腔注射荧光素：
依托 ORSO 系统的实时技术，您可使用清醒、自由活动的动物开展实验，开辟以往难以实现的全新研究方向。
生物现象始终处于动态变化，仅在固定时间点单次观测，往往无法完整反映整个过程，易造成数据解读偏差。会丢失快速动态变化过程中的信息。
清醒动物成像的全部优势：
- 麻醉操作耗时，会降低成像系统的实验通量。
- 麻醉会干扰生理状态、影响信号表达，带来实验偏差，直接改变动物模型的病理特征。（参考：Hildebrandt 等人，小动物活体成像中的麻醉及其他注意事项，ILAR，2008）
- 支持活体运动动物成像，可无创监测睡眠 - 觉醒周期内脑部活动，或是观测收缩肌肉中的生物发光信号，拓展研究边界。
With the real-time technology of the ORSO system, you can even perform experiments with non-anesthetized, freely moving animals, opening previously unimagined new opportunities for research.
生物现象始终处于动态变化，仅在固定时间点单次观测，往往无法完整反映整个过程，易造成数据解读偏差。会丢失快速动态变化过程中的信息。
清醒动物成像的全部优势：
- 麻醉操作耗时，会降低成像系统的实验通量。
- 麻醉会干扰生理状态、影响信号表达，带来实验偏差，直接改变动物模型的病理特征。（参考：Hildebrandt 等人，小动物活体成像中的麻醉及其他注意事项，ILAR，2008）
- 支持活体运动动物成像，可无创监测睡眠 - 觉醒周期内脑部活动，或是观测收缩肌肉中的生物发光信号，拓展研究边界。
ORSO 系统采用模块化设计理念，可紧跟成像技术的前沿进展；现有选配件模块以及未来新模块均可加装在仪器基础配置上。
光学成像操作简便：快速更换模块，在触摸屏上选择模块与采集模式，即可开始成像。
微距镜头模块
诸多实际研究场景，例如肿瘤研究中的细胞侵袭、细胞迁移实验，或是干细胞植入实验，都需要在不同空间分辨率下采集数据。微距镜头模块为此提供实用解决方案：可在宏观层面对整只动物无创追踪细胞；在器官或特定解剖位置评估细胞生长、扩散或迁移；也可在微观层面聚焦小细胞群。
微距镜头模块由 Biospace Lab 研发，为生物发光显微研究开辟新途径，填补荧光与生物发光成像在宏观成像和微观成像之间的技术空白。
微距镜头模块可实现离体样本高达 2.5 μm的高分辨率光学成像。可对感兴趣区域放大观测并完成精准定量，实现活体成像与离体成像结果关联分析。
诸多实际研究场景，例如肿瘤研究中的细胞侵袭、细胞迁移实验，或是干细胞植入实验，都需要在不同空间分辨率下采集数据。微距镜头模块为此提供实用解决方案：可在宏观层面对整只动物无创追踪细胞；在器官或特定解剖位置评估细胞生长、扩散或迁移；也可在微观层面聚焦小细胞群。
微距镜头模块由 Biospace Lab 研发，为生物发光显微研究开辟新途径，填补荧光与生物发光成像在宏观成像和微观成像之间的技术空白。
微距镜头模块可实现离体样本高达 2.5 μm的高分辨率光学成像。可对感兴趣区域放大观测并完成精准定量，实现活体成像与离体成像结果关联分析。
在行动中
In Actio 模块可同步采集生物发光信号以及红外照明下动物的明场视频，用于图像配准。将 ORSO 的成像能力从麻醉动物动态信号采集拓展至自由活动动物的动态信号采集。在不需要 3D 模块提供多视角成像的场景下，In Actio 模块具备以下优势：
提升生理相关性：观测不受麻醉干扰的生理现象。
缩短动物前处理时间：无需麻醉，降低动物应激反应。
更高实验通量：省去麻醉步骤，实验流程更快，可同时对多只动物成像。
4VIEW – 3D
4VIEW 3D 模块可实现光学信号更优的定位与定量分析。.
X 射线模块
X 射线模块支持将 X 射线影像与光学信号图像叠加融合。借助这套高分辨率解剖结构信息，可轻松完成光学信号的精准定位。
相比其他手段，X 射线模块能够实现光学信号更优的解剖学定位。示例展示可同时对至多 5 只小鼠开展低剂量成像。
X 射线模块
X 射线模块支持将 X 射线影像与光学信号图像叠加融合。借助这套高分辨率解剖结构信息，可轻松完成光学信号的精准定位。
相比其他手段，X 射线模块能够实现光学信号更优的解剖学定位。示例展示可同时对至多 5 只小鼠开展低剂量成像。
TOMOFLUO
TomoFluo 模块专为活体荧光信号的高精度三维定量而研发。该模块采用新一代活体荧光断层成像算法 fDOT。fDOT（荧光扩散光学断层成像）依托迭代代数重建技术（ART），降低散射与衰减伪影，同时改善样本边界相关问题。三维荧光体数据可叠加至动物三维表面重建模型，也能够与 CT/PET/SPECT/MRI 影像配准融合。
TomoFluo 模块可对深部组织信号进行定量，实现精准药代动力学分析与长时程动态定量检测。
短波红外成像（SWIR / NIR II）
专用NIR-II成像，保障出色的SWIR成像性能
可在短波红外波段（SWIR）采集图像，具备更深的组织穿透能力、高灵敏度与高空间分辨率。
- 降低组织自发荧光带来的干扰
- 血液与组织对光吸收更少
- 降低光散射效应
ORIA
ORIA 是 Biospace Lab 专为NIR-II / SWIR荧光成像打造的光学成像系统，光谱覆盖 900–1700 nm波段。 NIR-II / SWIR fluorescence imaging, covering the 900–1700 nm spectral range.
实现光子深度穿透是光学成像的核心目标之一。与近红外一区（NIR-I，600–900 nm）相比，NIR II与NIR III的特点是组织自发荧光更低、光散射更少、光吸收更弱。SWIR具备高空间分辨率，加之极低的光吸收与自发荧光带来的高灵敏度，可实现更深层、更细小血管的可视化与动态观测。
ORIA 系统灵敏度高，可实现高帧率采集，是实时成像的理想方案。可高精度非接触式监测呼吸与心率，同时开展实时代谢成像。
纳米测温是SWIR成像独具特色的核心应用之一，依托纳米测温探针这类专用纳米颗粒的光谱特征实现检测。纳米测温应用包含肿瘤早期检测、酶活性监测、药物疗效评估以及热疗过程管控。随着纳米测温探针持续迭代开发，温度传感技术不断发展，能够获取全新且不可或缺的生物学信息。
Biospace Lab 是唯一可在 ORIA 系统上配套集成纳米测温模块的厂商。
监测目标区域温度，获取更多实验数据，支持以下三种纳米测温采集模式：
- 时序荧光成像
- 双通道时序荧光成像（比率法） sequential Fluorescence Imaging (Ratiometry)
- 通过荧光寿命成像抑制组织本征自发荧光，进一步提升检测灵敏度。 fluorescence lifetime imaging.
Contact us to customize your SWIR instrument for your applications of interest. Choose among different lasers, filters, cameras, etc.
放射自显影
可实现超快速定量成像，检测低能放射性同位素及多种同位素在微观尺度下的生物分布。
BETAIMAGER系统
无论您需要高速采集还是高分辨率成像，Biospace Lab 都可为您的放射自显影应用提供理想方案。
BETAIMAGER TRACER
The BetaIMAGER™ tRACER is the system of choice for applications requiring the highest sensitivity and either high throughput, or larger sample sizes. With the BetaIMAGER tRACER, autoradiography becomes a fast and easy routine.
BetaIMAGER tRACER 是高灵敏度、大通量或大样本检测场景的优选设备。借助 BetaIMAGER tRACER，放射自显影可成为高效简便的常规实验手段。氚是生物样本检测中常用的 β 放射源，但传统检测速度很慢。传统胶片与磷屏成像方法往往需要数周时间。如今 BetaIMAGER tRACER 系统提供更优方案，可将氚成像时间从数周缩短至数小时，满足实验室大通量需求。针对超低能量的氚射线，设备仍具备优异灵敏度：tRACER 系统可检测至 0.007 cpm/mm²的氚活度。
BetaIMAGER 视野规格可达 200 × 250 mm，可同时放置不超过 15 张显微镜载玻片、多块大组织切片或薄层色谱板，同步完成成像采集。
根据左侧图中采集结果计算样本活度（覆盖完整样本）：7.5 × 10³ hpm（击中数 / 分钟），折合 6.82 nCi。
The BetaIMAGER™ tRACER is the system of choice for applications requiring the highest sensitivity and either high throughput, or larger sample sizes. With the BetaIMAGER tRACER, autoradiography becomes a fast and easy routine.
Tritium is a reliable beta emitter for measurement of biological samples. The big problem is that detection is slow. Traditional film and phosphor imaging methods can take weeks to complete. But now, there’s a better way to meet the high throughput needs of your lab: The BetaIMAGER tRACER system cuts your tritium imaging time from weeks to hours. Exceptional sensitivity can be obtained even with ultra-low energy tritium emissions: the tRACER system detects tritium levels as low as 0.007 cpm/mm2.
BetaIMAGER 视野规格可达 200 × 250 mm，可同时放置不超过 15 张显微镜载玻片、多块大组织切片或薄层色谱板，同步完成成像采集。
Sample activity was calculated using the acquisition shown in the figure on the left (exhibits the entire sample): 7.5 x 10^3 hpm (hits per minute) which translates to 6.82 nCi.
BETAIMAGER DFINE
数字放射自显影，具备优异的空间分辨率。
BetaIMAGER DFINE 适用于小组织切片、对空间分辨率有极高要求的实验场景。自动载物台可放置 4 份样本，单份样本尺寸可达 24 × 32 mm，依次完成成像采集。检测氚样本时，空间分辨率可达 10 μm。DFINE 是 PET、SPECT 活体分子成像理想的离体配套设备。
针对氚标记样本，BetaIMAGER DFINE 成像速度是胶片法的 50 倍，可实现精确定量。DFINE 检测氚标记样本分辨率可达 10 μm，在非胶片类技术中性能领先。此外，DFINE 操作简单，无需学习复杂技术与流程。
The ONLY high-resolution digital autoradiography solution.
The BetaIMAGER™ DFINE is the system of choice for applications involving smaller tissue sections and where the best possible spatial resolution is required. Four samples, up to 24 x 32 mm each in size, can be loaded onto the automated stage for sequential image acquisition. Spatial resolution as high as 10 μm can be achieved with tritium. The DFINE is the perfect ex-vivo complement to PET and SPECT molecular imaging.
针对氚标记样本，BetaIMAGER DFINE 成像速度是胶片法的 50 倍，可实现精确定量。DFINE 检测氚标记样本分辨率可达 10 μm，在非胶片类技术中性能领先。此外，DFINE 操作简单，无需学习复杂技术与流程。
如何选择适合您的 BetaIMAGER？
您可根据需求选择：高灵敏度与大通量版本，或是超高空间分辨率版本。
Why digital autoradiography ?
Linear dynamic range of the BetaIMAGER
Courtesy of Dr Alain Schweizer, Novartis
The BetaIMAGER systems count all emitted particles, regardless of their energy. This direct beta counting technology of the BetaIMAGER systems provides unequalled performance: the BetaIMAGER tRACER system is approximately 500X more sensitive and the BetaIMAGER dFINE system about 50X more sensitive to tritium than either X-ray film or storage phosphor screen. The BetaIMAGER tRACER system is unique in its ability to detect tritium levels as low as 0.007 cpm/mm2.
The BetaIMAGER systems provide precise localization and quantification of beta-emitting radioligands. The advanced image analysis tools of the M3Vision software allow for direct analysis of digital data and a better than 1:10,000 linear dynamic range ensuring accurate quantification over the widest possible range.
You can detect and quantify both high and low sample concentrations in a single data acquisition.
Biospace Lab developed an exclusive technology for the simultaneous detection and discrimination of multiple radioisotopes.
A single sample can be simultaneously labeled with several radioisotopes and the specific contribution from each label can be detected during a single data acquisition. Discrimination can be based on emission energy (2 radioisotopes), or on radioactive half-life (2 isotopes or more). Examples of dual-detection radioisotope pairs include 3H/14C, 3H/35S, 3H/32P or 33P and an example of a multi-label combination would be 99mTc/ 111In/18F.
This unique radioisotope discrimination feature allows the development of more rapid and accurate protocols for recording the distribution of several molecules simultaneously in a single specimen.
You can determine co-localization of different receptors, or receptors and ligands; Map complex functional activity with pathology; or Compare specific gene expression with a reference gene.
The exclusive real-time detection technology used in both systems displays and accumulates counts in real-time:
Real-time autoradiography: distribution of D1 receptors in rat brain striatum following tritiated ligand binding
X-Ray film or storage phosphor screen-based autoradiography requires a two-step blind acquisition process: expose and then scan
The real-time acquisition allows the instantaneous display of the image recorded by the camera and to follow the progression of the reconstitution of the final image. The image display is refreshed every 30 seconds allowing the user to evaluate it during the acquisition.
The unmatched sensitivity reduces your data acquisition time from weeks to hours.
You can see data accumulating in real-time and obtain the first results in minutes.
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UNIQUE SYSTEM DETECTS AND QUANTIFIES BOTH HIGH AND LOW SAMPLE CONCENTRATIONS IN A SINGLE DATA ACQUISITION WITH BOTH HIGH SENSITIVITY AND RESOLUTION.
The BetaIMAGER™ systems count all emitted particles, regardless of their energy. This direct beta counting technology of the BetaIMAGER systems provides unequalled performance:
the BetaIMAGER tRACER system is approximately 500X more sensitive and the BetaIMAGER dFINE system about 50X more sensitive to tritium than either X-ray film or Storage Phosphor Screen. The BetaIMAGER™ tRACER system is unique in its ability to detect tritium levels as low as 0.007 cpm/mm2.
The BetaIMAGER systems provide precise localization and quantification of beta emitting radioligands. The advanced image analysis tools of the M3 vision software allow for direct analysis of digital data and a better than 1:10,000 linear dynamic range ensuring accurate quantification over the widest possible range.
Linear dynamic range of the BetaIMAGER
Courtesy of Dr Alain Schweizer, Novartis
You can detect and quantify both high and low sample concentrations in a single data acquisition.
Biospace Lab developed an exclusive technology for the simultaneous detection and discrimination of multiple radioisotopes
A single sample can be simultaneously labeled with several radioisotopes and the specific contribution from each label can be detected during a single data acquisition. Discrimination can be based on emission energy (2 radioisotopes), or on radioactive half-life (2 isotopes or more). Examples of dual-detection radioisotope pairs include 3H/14C,3H/35S, 3H/32P or 33P and an example of a multi-label combination would be 99mTc/ 111In/18F
This unique radioisotope discrimination feature allows the development of more rapid and accurate protocols for recording the distribution of several molecules simultaneously in a single specimen.
You can determine co-localization of different receptors, or receptors and ligands; Map complex functional activity with pathology; or Compare specific gene expression with a reference gene.
Disintegrations are detected in real-time and the data display is continuously updated during acquisition. Under or overexposure of the sample are eliminated.
Longer acquisitions deliver higher image dynamics. The absence of limit on the duration of the acquisition allows the detection of weak signals and the improvement of resolution.
Thanks to their high sensitivity, BetaIMAGER systems can detect ultra-low levels of radiolabeled ligands in the sample. You can use minimal amounts of radiolabeled compounds and reduce radioactive waste as well as minimize your experimental costs.
Reduce the number of experiments thanks to the reproducibility of the system.
Non-destructive imaging and measurement: specimens are never damaged or destroyed during the process.
软件
The IMAGER systems are driven by the PhotoAcquisition and BetaAcquisition software application, which seamlessly control the entire system and its modules, and manage the imaging experiments.
Simple and user-friendly acquisition software.
Immediate visualization from the beginning of acquisition is one of the hallmarks making real-time imaging so practical. You can see the first signals after just a few seconds and can quickly stop the acquisition or extend it knowing that the signal will continue to accumulate until the desired endpoint.
Real-time feedback allows for optimized and automated control of some acquisition parameters such as the opening of the lens during the acquisition according to signal progression. These automatic time optimization capabilities simplify the use without blind configuration of the lens opening, integration time or binning level which must often be determined experimentally on the cooled CCD camera systems.
M3Vision analysis software allows users to visualize 2D and 3D images, freely adjust the signal display threshold, and perform quantifications based on desired ROIs such as geometrical forms or signal intensity thresholds.
ROI measurements provide total, maximum, minimum and average signal intensity values.
M3Vision makes time analysis possible, allowing quantifications at multiple ROIs at the plateau time. For high speed studies, an automatic selection tool of the temporal window to the signal plateau enables rapid analysis of each ROI.
Export all data, measurements and time profiles to Excel in a single click! For the longitudinal studies, all acquisitions linked to a study can be exported in a single Excel workbook that includes one tab per acquisition as well as a summary table of all the quantifications.
Software analysis tools include numerous functions to allow precise and automatic quantification:
- The magic wand tool allows you to automatically draw ROIs determined by signal intensity thresholds. These ROIs can then be created and analyzed automatically by the software in a fraction of a second.
- Automatic temporal analysis enables automated, streamlined and rapid quantification of each ROI in just a few seconds.
When an acquisition is saved, ALL acquisition settings (including lens opening, plate position, acquisition time, grid speed, date and time) are also saved. User-generated comments can also be saved such as observations about the animals being imaged, and notable events that occurred before or during acquisition. This information is linked to the acquisition folder and is accessible through the analysis software.
M3Vision is a licensed software.